Notice: Undefined index: linkPowrot in C:\wwwroot\wwwroot\publikacje\publikacje.php on line 1275
Publikacje
Pomoc (F2)
[55190] Artykuł:

The role of Proteus mirabilis cell wall features in biofilm formation

Czasopismo: Archives of Microbiology   Tom: 198(9), Zeszyt: 9, Strony: 877-884
ISSN:  0302-8933
Wydawca:  SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA
Opublikowano: Pażdziernik 2016
 
  Autorzy / Redaktorzy / Twórcy
Imię i nazwisko Wydział Katedra Procent
udziału
Liczba
punktów
Grzegorz Czerwonka11.00  
Anna Guzy11.00  
Klaudia Kałuża11.00  
Michalina Grosicka11.00  
Magdalena Dańczuk orcid logoWiŚGiEKatedra Technologii Wody i Ścieków1120.00  
Łukasz Lechowicz11.00  
Dawid Gmiter11.00  
Paweł Kowalczyk11.00  
Wiesław Kaca11.00  

Grupa MNiSW:  Publikacja w czasopismach wymienionych w wykazie ministra MNiSzW (część A)
Punkty MNiSW: 20
Klasyfikacja Web of Science: Article


Pełny tekstPełny tekst     DOI LogoDOI     Web of Science Logo Web of Science    
Keywords:

Proteus mirabilis  biofilm  electrokinetic potential  epifluorescence microscopy  Cell surface  hydrophobicity CSH 



Abstract:

Biofilms formed by Proteus mirabilis strains are a serious medical problem, especially in the case of urinary tract infections. Early stages of biofilm formation, such as reversible and irreversible adhesion, are essential for bacteria to form biofilm and avoid eradication by antibiotic therapy. Adhesion to solid surfaces is a complex process where numerous factors play a role, where hydrophobic and electrostatic interactions with solid surface seem to be substantial. Cell surface hydrophobicity and electrokinetic potential of bacterial cells depend on their surface composition and structure, where lipopolysaccharide, in Gram-negative bacteria, is prevailing. Our studies focused on clinical and laboratory P. mirabilis strains, where laboratory strains have determined LPS structures. Adherence and biofilm formation tests revealed significant differences between strains adhered in early stages of biofilm formation. Amounts of formed biofilm were expressed by the absorption of crystal violet. Higher biofilm amounts were formed by the strains with more negative values of zeta potential. In contrast, high cell surface hydrophobicity correlated with low biofilm amount.