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[55190] Artykuł: The role of Proteus mirabilis cell wall features in biofilm formationCzasopismo: Archives of Microbiology Tom: 198(9), Zeszyt: 9, Strony: 877-884ISSN: 0302-8933 Wydawca: SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA Opublikowano: Pażdziernik 2016 Autorzy / Redaktorzy / Twórcy Grupa MNiSW: Publikacja w czasopismach wymienionych w wykazie ministra MNiSzW (część A) Punkty MNiSW: 20 Klasyfikacja Web of Science: Article Pełny tekst DOI Web of Science Keywords: Proteus mirabilis  biofilm  electrokinetic potential  epifluorescence microscopy  Cell surface  hydrophobicity CSH  |
Biofilms formed by Proteus mirabilis strains are a serious medical problem, especially in the case of urinary tract infections. Early stages of biofilm formation, such as reversible and irreversible adhesion, are essential for bacteria to form biofilm and avoid eradication by antibiotic therapy. Adhesion to solid surfaces is a complex process where numerous factors play a role, where hydrophobic and electrostatic interactions with solid surface seem to be substantial. Cell surface hydrophobicity and electrokinetic potential of bacterial cells depend on their surface composition and structure, where lipopolysaccharide, in Gram-negative bacteria, is prevailing. Our studies focused on clinical and laboratory P. mirabilis strains, where laboratory strains have determined LPS structures. Adherence and biofilm formation tests revealed significant differences between strains adhered in early stages of biofilm formation. Amounts of formed biofilm were expressed by the absorption of crystal violet. Higher biofilm amounts were formed by the strains with more negative values of zeta potential. In contrast, high cell surface hydrophobicity correlated with low biofilm amount.